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chk2 t68p  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc chk2 t68p
    Chk2 T68p, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chk2+t68p/us12594293-489-17-23
    Average 86 stars, based on 1 article reviews
    chk2 t68p - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Western Blot:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Control:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Irradiation:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Positive Control:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Phospho-proteomics:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Software:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Immunostaining:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Transfection:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Flow Cytometry:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).

    Staining:

    Article Title: Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
    Article Snippet: blot analysis was performed as described previously (22). .. Antibodies used were against ATM-S1981P (R&D Systems); ATM (SIGMA); ATR-T1989P (GeneTex); and ATR, CHK1-S345P, CHK1, CHK2-T68P, CHK2, cleaved caspase-3, and b-actin (Cell Signaling Technology). .. Bands were visualized using ECL with Azurec500 imaging system (Az

    Article Title: Hyperactivation of DNA-PK by Double-Strand Break Mimicking Molecules Disorganizes DNA Damage Response
    Article Snippet: Rabbit polyclonal antibodies against the following targets were used: Rad51 (Calbiochem, San Diego, California, USA), Nbs1, Rpa32-S4/8P (Novus Biologicals, Littleton, CO, USA), 53BP1, H2AX, Chk2-T68P, p53-S15P, Nbs1-S343P (Cell Signaling Technology) DNA-PKcs-S2056P (generous gift of David.. J. Chen).



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    Figure 8. Expression of RFTS induces various DNA damage responses. (A) WT-11 cells grown on cov- erslips were uninduced (+Dox) or induced (-Dox) for 72 hours. The cells were then immunostained with antibodies against phospho-ATM-Serine 1981 (ATM-S1981P), 53BP1, and <t>phospho-Chk2-Threonine</t> 68 <t>(Chk2-T68P),</t> respectively. (Scale bar: 10 μm.) (B) Quantification of foci positive (>5 foci/cell) cells from (A). Results showed the means ± SEM from 2 independent experiments. (C) Cell extracts from Figure 7B were separated by SDS-PAGE and immunoblotted with antibody against phospho-p53-serine 15 (p53- S15P) and PCNA.
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    Image Search Results


    Figure 8. Expression of RFTS induces various DNA damage responses. (A) WT-11 cells grown on cov- erslips were uninduced (+Dox) or induced (-Dox) for 72 hours. The cells were then immunostained with antibodies against phospho-ATM-Serine 1981 (ATM-S1981P), 53BP1, and phospho-Chk2-Threonine 68 (Chk2-T68P), respectively. (Scale bar: 10 μm.) (B) Quantification of foci positive (>5 foci/cell) cells from (A). Results showed the means ± SEM from 2 independent experiments. (C) Cell extracts from Figure 7B were separated by SDS-PAGE and immunoblotted with antibody against phospho-p53-serine 15 (p53- S15P) and PCNA.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: Expression of PCNA-binding domain of CtIP, a motif required for CtIP localization at DNA replication foci, causes DNA damage and activation of DNA damage checkpoint.

    doi: 10.4161/cc.8.9.8322

    Figure Lengend Snippet: Figure 8. Expression of RFTS induces various DNA damage responses. (A) WT-11 cells grown on cov- erslips were uninduced (+Dox) or induced (-Dox) for 72 hours. The cells were then immunostained with antibodies against phospho-ATM-Serine 1981 (ATM-S1981P), 53BP1, and phospho-Chk2-Threonine 68 (Chk2-T68P), respectively. (Scale bar: 10 μm.) (B) Quantification of foci positive (>5 foci/cell) cells from (A). Results showed the means ± SEM from 2 independent experiments. (C) Cell extracts from Figure 7B were separated by SDS-PAGE and immunoblotted with antibody against phospho-p53-serine 15 (p53- S15P) and PCNA.

    Article Snippet: The antibodies used for immunostaining include anti-CtIP (K19, Santa Cruz Biotechnology), anti-PCNA (Ab-1, Oncogene), antiBrdU (Amersham-Pharmacia, RPN202), anti-γ-H2AX (Upstate Biotechnology), anti-ATM-S1981P (Upstate Biotechnology) and anti-Chk2 T68P (Cell Signaling Technology).

    Techniques: Expressing, SDS Page